Spinach-based RNA mimicking GFP in plant cells.

Spinach RNA-mimicking GFP (S-RMG) has been successfully used to monitor cellular RNAs including microRNAs in bacterium, yeast, and human cells. However, S-RMG has not been established in plants. In this study, we found that like bacterial, yeast, and human cellular tRNAs, plant tRNAs such as tRNALys can protect and/or stabilize the Spinach RNA aptamer interaction with the fluorophore DFHBI enabling detectable levels of green fluorescence to be emitted. The tRNALys-Spinach-tRNALys, once delivered into "chloroplast-free" onion epidermal cells can emit strong green fluorescence in the presence of DFHBI. Our results demonstrate for the first time that Spinach-based RNA visualization has the potential for in vivo monitoring of RNAs in plant cells. © 2022. The Author(s).

Citation

Zhiming Yu, Yue Wang, Fengling Mei, Haiting Yan, Zhenhui Jin, Pengcheng Zhang, Xian Zhang, Mahmut Tör, Stephen Jackson, Nongnong Shi, Yiguo Hong. Spinach-based RNA mimicking GFP in plant cells. Functional & integrative genomics. 2022 Jun;22(3):423-428

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PMID: 35267109

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