Novel, Inexpensive, and Scalable Amyloid Fibril Formation Method.

Wheat flour was used as a source of protein for the in vitro synthesis of Amyloid fibrils to develop a novel and inexpensive fabrication method. Amyloid fibrillation was confirmed by Thioflavin T Fluorescence, using confocal microscopy. A morphological study was carried out by transmission electron microscopy (TEM), which revealed the high aspect ratio of the amyloid fibrils formed via a novel process. An application of the amyloid fibers produced by the novel method is shown to be melatonin sensing. Tests showed that the amyloid samples had a measurable color variation dependent on the melatonin concentration. This newly derived process could prove to be a cost-effective tool for future nano-biomaterial applications in commercial and research settings.

Citation

Ethan Hessick, Milind Pawar, Reid Souchereau, Emma Schmitz, Pelagia-Irene Gouma. Novel, Inexpensive, and Scalable Amyloid Fibril Formation Method. Materials (Basel, Switzerland). 2022 Feb 26;15(5)

PMID: 35268997

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