The structural basis for regulation of the glutathione transporter Ycf1 by regulatory domain phosphorylation.

Yeast Cadmium Factor 1 (Ycf1) sequesters heavy metals and glutathione into the vacuole to counter cell stress. Ycf1 belongs to the ATP binding cassette C-subfamily (ABCC) of transporters, many of which are regulated by phosphorylation on intrinsically-disordered domains. The regulatory mechanism of phosphorylation is still poorly understood. Here, we report two cryo-EM structures of Ycf1 at 3.4 Å and 4.0 Å resolution in inward-facing open conformations that capture previously unobserved ordered states of the intrinsically disordered regulatory domain (R-domain). R-domain phosphorylation is clearly evident and induces a topology promoting electrostatic and hydrophobic interactions with Nucleotide Binding Domain 1 (NBD1) and the Lasso motif. These interactions stay constant between the structures and are related by rigid body movements of the NBD1/R-domain complex. Biochemical data further show R-domain phosphorylation reorganizes the Ycf1 architecture and is required for maximal ATPase activity. Together, we provide insights into how R-domains control ABCC transporter activity. © 2022. The Author(s).

Citation

Nitesh Kumar Khandelwal, Cinthia R Millan, Samantha I Zangari, Samantha Avila, Dewight Williams, Tarjani M Thaker, Thomas M Tomasiak. The structural basis for regulation of the glutathione transporter Ycf1 by regulatory domain phosphorylation. Nature communications. 2022 Mar 11;13(1):1278

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PMID: 35277487

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