A "turn-on" DNA-scaffolded silver-nanocluster probe for detection of tumor-related mRNA.

Jingjing Gao, Hui Xu, Faju Hou, Shengxiao Zhang

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry 2022 Feb

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A novel and "turn-on" DNA-silver nanocluster probe (DNA/AgNCs) was developed that can detect the level of breast tumor-related mRNA markers: cyclin D1 mRNA. The fluorescence of DNA/AgNCs probe rendered a "turn-on" response based on the secondary structure changes induced by the target strand. In the presence of target chain, the fluorescence enhances in a concentration-dependent manner, which enables the quantitative detection of analytes. By this method, a detection limit of 2.1 nM and a linear range of 15-125 nM were achieved. In addition, the probe displays excellent discrimination between a perfectly complementary target and single-base mismatch targets, providing an alternative approach for quick and specific recognition and quantification of mRNA or DNA. © 2022. The Author(s), under exclusive licence to The Japan Society for Analytical Chemistry.

Citation

Jingjing Gao, Hui Xu, Faju Hou, Shengxiao Zhang. A "turn-on" DNA-scaffolded silver-nanocluster probe for detection of tumor-related mRNA. Analytical sciences : the international journal of the Japan Society for Analytical Chemistry. 2022 Feb;38(2):419-426