Characterization of DNA-PK-Bound End Fragments Using GLASS-ChIP.

Rajashree A Deshpande, Tanya T Paull

Methods in molecular biology (Clifton, N.J.) 2022

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Endonucleolytic cleavage of DNA ends by the human Mre11-Rad50-Nbs1 (MRN) complex occurs in a manner that is promoted by DNA-dependent protein kinase (DNA-PK). A method is described to isolate DNA-PK-bound fragments released from chromatin in human cells using a modified Gentle Lysis and Size Selection chromatin immunoprecipitation (GLASS-ChIP) protocol. This method, combined with real-time PCR or next-generation sequencing, can identify sites of MRN endonucleolytic cutting adjacent to DNA-PK binding sites in human cells. © 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Citation

Rajashree A Deshpande, Tanya T Paull. Characterization of DNA-PK-Bound End Fragments Using GLASS-ChIP. Methods in molecular biology (Clifton, N.J.). 2022;2444:171-182