Long-term effect of perfluorooctanoic acid on the anammox system based on metagenomics: Performance, sludge characteristic and microbial community dynamic.

Linqin Tang, Chengyuan Su, Cuiping Fan, Ruting Li, Yuchen Wang, Shu Gao, Menglin Chen

Bioresource technology 2022 May

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The effects of PFOA on the nitrogen removal performance, microbial community and functional genes of anaerobic ammonium oxidation (anammox) sludge in an anaerobic baffled reactor (ABR) were investigated. The removal efficiencies of ammonia nitrogen (NH4+-N) and nitrite (NO2--N) decreased from 93.90 ± 3.64% and 98.6 ± 1.84% to 77.81 ± 6.86% and 77.96 ± 1.88% when PFOA increased from 5 mg/L to 50 mg/L, respectively. X-ray photoelectron spectra analysis of the anammox sludge showed the presence of both C-F and CaF2 forms of F. Metagenomics analysis of the anammox sludge in the first compartment illustrated that the relative abundance of Ca.Brocadia and Ca.Kuenenia decreased from 22.21% and 5.61% to 2.11% and 2.84% at 50 mg/L PFOA compared with that without PFOA. In addition, the nitrogen metabolism pathway showed that adding 50 mg/L PFOA decreased the expression of HzsB, HzsC, and Hdh (anammox genes) by 0.096%, 0.05% and 0.062%, respectively. Copyright © 2022 Elsevier Ltd. All rights reserved.

Citation

Linqin Tang, Chengyuan Su, Cuiping Fan, Ruting Li, Yuchen Wang, Shu Gao, Menglin Chen. Long-term effect of perfluorooctanoic acid on the anammox system based on metagenomics: Performance, sludge characteristic and microbial community dynamic. Bioresource technology. 2022 May;351:127002