Correlation Engine 2.0
Clear Search sequence regions

  • Cdc7 (6)
  • cell cycle (2)
  • Dbf4 (6)
  • displaces (1)
  • electron (1)
  • kinases (2)
  • MCM (5)
  • Mcm2 (1)
  • Mcm4 (3)
  • Mcm6 (2)
  • regulates (1)
  • rich (2)
  • subunit (2)
  • yeast (1)
  • Sizes of these terms reflect their relevance to your search.

    The Dbf4-dependent kinase Cdc7 (DDK) regulates DNA replication initiation by phosphorylation of the MCM double hexamer (MCM-DH) to promote helicase activation. Here, we determine a series of cryo electron microscopy (cryo-EM) structures of yeast DDK bound to the MCM-DH. These structures, occupied by one or two DDKs, differ primarily in the conformations of the kinase core. The interactions of DDK with the MCM-DH are mediated exclusively by subunit Dbf4 straddling across the hexamer interface on the three N-terminal domains (NTDs) of subunits Mcm2, Mcm6, and Mcm4. This arrangement brings Cdc7 close to its only essential substrate, the N-terminal serine/threonine-rich domain (NSD) of Mcm4. Dbf4 further displaces the NSD from its binding site on Mcm4-NTD, facilitating an immediate targeting of this motif by Cdc7. Moreover, the active center of Cdc7 is occupied by a unique Dbf4 inhibitory loop, which is disengaged when the kinase core assumes wobbling conformations. This study elucidates the versatility of Dbf4 in regulating the ordered multisite phosphorylation of the MCM-DH by Cdc7 kinase during helicase activation. © 2022. The Author(s).


    Jiaxuan Cheng, Ningning Li, Yunjing Huo, Shangyu Dang, Bik-Kwoon Tye, Ning Gao, Yuanliang Zhai. Structural Insight into the MCM double hexamer activation by Dbf4-Cdc7 kinase. Nature communications. 2022 Mar 16;13(1):1396

    Expand section icon Mesh Tags

    Expand section icon Substances

    PMID: 35296675

    View Full Text