Cryo-EM Structure Reveals Polymorphic Ligand-bound States of IGF1R.

Xi Zhang, Tianzi Wei, Cang Wu, Junyi Jiang, Shengming Chen, Yinqing Hu, Yi Lu, Dayong Sun, Liting Zhai, Jian Zhang, Chuang Liu

Journal of molecular biology 2022 May 15

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Type 1 insulin-like growth factor receptor (IGF1R) plays an important role in regulating cellular metabolism and cell growth and has been identified as an anticancer drug target. Although previous studies have revealed some structures of IGF1R with different ligands, the continuous dynamic conformation change remains unclear. Here, we report 10 distinct structures (7.9-3.6 Å) of IGF1R bound to IGF1 or insulin to reveal the polymorphic conformations of ligand-bound IGF1R. These results showed that the α-CT2, disulfide bond (C670-C670'), and FnIII-2 domains had the most flexible orientations for the conformational change that occurs when ligands bind to the receptor. In addition, we found one special conformation (tentatively named the diverter-switch state) in both complexes, which may be one of the apo-IGF1R forms under ligand-treatment conditions. Hence, these results illustrated the mechanism of how different ligands could bind to human IGF1R and provided a rational template for drug design. Copyright © 2022 Elsevier Ltd. All rights reserved.

Citation

Xi Zhang, Tianzi Wei, Cang Wu, Junyi Jiang, Shengming Chen, Yinqing Hu, Yi Lu, Dayong Sun, Liting Zhai, Jian Zhang, Chuang Liu. Cryo-EM Structure Reveals Polymorphic Ligand-bound States of IGF1R. Journal of molecular biology. 2022 May 15;434(9):167536