Correlation Engine 2.0
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    Despite the importance of cilia in cell signaling and motility, the molecular mechanisms regulating cilium formation remain incompletely understood. Herein, we characterize enkurin domain-containing protein 1 (ENKD1) as a novel centrosomal protein that mediates the removal of centriolar coiled-coil protein 110 (CP110) from the mother centriole to promote ciliogenesis. We show that Enkd1 knockout mice possess ciliogenesis defects in multiple organs. Super-resolution microscopy reveals that ENKD1 is a stable component of the centrosome throughout the ciliogenesis process. Simultaneous knockdown of ENKD1 and CP110 significantly reverses the ciliogenesis defects induced by ENKD1 depletion. Protein interaction analysis shows that ENKD1 competes with centrosomal protein 97 (CEP97) in binding to CP110. Depletion of ENKD1 enhances the CP110-CEP97 interaction and detains CP110 at the mother centriole. These findings thus identify ENKD1 as a centrosomal protein and uncover a novel mechanism controlling CP110 removal from the mother centriole for the initiation of ciliogenesis. © 2022 The Authors.


    Ting Song, Yunfan Yang, Peng Zhou, Jie Ran, Liang Zhang, Xiaofan Wu, Wei Xie, Tao Zhong, Hongbin Liu, Min Liu, Dengwen Li, Huijie Zhao, Jun Zhou. ENKD1 promotes CP110 removal through competing with CEP97 to initiate ciliogenesis. EMBO reports. 2022 May 04;23(5):e54090

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    PMID: 35301795

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