Qiaofeng Li, Tieqiang Sun, Gert Ij Salentijn, Baoan Ning, Dianpeng Han, Jialei Bai, Yuan Peng, Zhixian Gao, Zhouping Wang
Journal of hazardous materials 2022 Jun 15Polydiacetylene (PDA) is very suited for sensitively detecting large biomolecules, and its unique chromatic properties enable visual read-out. However, application to the selective detection of small molecules remains challenging. Here, bifunctional ligands are studied to amplify the color change of PDA for biorecognition of small molecules for the smartphone-based detection of diethylstilbestrol (DES). PDA is decorated with streptavidin (PDA-SA, blue), and biotin-modified DES (bio-DES) is prepared as a bifunctional ligand to couple with PDA-SA and DES antibody. Since multiple bio-DES can bind to a single SA, then multiple SAs on PDA lead to an increased surface coverage of the vesicle. In samples without DES, PDA-SA-bio-DES-DES antibody complexes will form, leading to a color transition (blue to red); this color transition is greatly amplified by antibody-induced aggregation of the complexes. When DES is present, aggregation is inhibited due to competition for the antibody and PDA-SA-bio-DES retains its blue color. A linear relationship (0.4-1250 ng mL-1) is found between the colorimetric response and the logarithmic DES concentration, with adequate selectivity, accuracy (82.24-118.64%), and precision (below 8.24%). Finally, a paper-based DES PDA biosensor is developed with visual and smartphone-based detection limits of 10 ng mL-1 and 0.85 ng mL-1 in water, respectively. Copyright © 2022 Elsevier B.V. All rights reserved.
Qiaofeng Li, Tieqiang Sun, Gert Ij Salentijn, Baoan Ning, Dianpeng Han, Jialei Bai, Yuan Peng, Zhixian Gao, Zhouping Wang. Bifunctional ligand-mediated amplification of polydiacetylene response to biorecognition of diethylstilbestrol for on-site smartphone detection. Journal of hazardous materials. 2022 Jun 15;432:128692
PMID: 35316640
View Full Text