Novel indole-mediated potassium ion import system confers a survival advantage to the Xanthomonadaceae family.

Yuxiang Zhu, Yong Han, Guanglei Liu, Zeran Bian, Xiayi Yan, Yaoyao Li, Hongan Long, Guanshuo Yu, Yan Wang

The ISME journal 2022 Jul

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Interspecific and intraspecific communication systems of microorganisms are involved in the regulation of various stress responses in microbial communities. Although the significance of signaling molecules in the ubiquitous family Xanthomonadaceae has been reported, the role bacterial communications play and their internal mechanisms are largely unknown. Here, we use Lysobacter enzymogenes, a member of Xanthomonadaceae, to identify a novel potassium ion import system, LeKdpXFABC. This import system participates in the indole-mediated interspecies signaling pathway and matters in environmental adaptation. Compared with the previously reported kdpFABC of Escherichia coli, LekdpXFABC contains a novel indispensable gene LekdpX and is directly regulated by the indole-related two-component system QseC/B. QseC autophosphorylation is involved in this process. The operon LekdpXFABC widely exists in Xanthomonadaceae. Moreover, indole promotes antimicrobial product production at the early exponential phase. Further analyses show that indole enhances potassium ion adsorption on the cell surface by upregulating the production of O-antigenic polysaccharides. Finally, we confirm that LeKdpXFABC mediation by indole is subject to the intraspecific signaling molecules DSFs, of which the biosynthesis genes always exist together with LekdpXFABC. Therefore, as a new idea, the signal collaborative strategy of indole and DSFs might ensure the persistent fitness advantage of Xanthomonadaceae in variable environments. © 2022. The Author(s), under exclusive licence to International Society for Microbial Ecology.

Citation

Yuxiang Zhu, Yong Han, Guanglei Liu, Zeran Bian, Xiayi Yan, Yaoyao Li, Hongan Long, Guanshuo Yu, Yan Wang. Novel indole-mediated potassium ion import system confers a survival advantage to the Xanthomonadaceae family. The ISME journal. 2022 Jul;16(7):1717-1729