Barley stripe mosaic virus γb protein targets thioredoxin h-type 1 to dampen salicylic acid-mediated defenses.

Zhihao Jiang, Xuejiao Jin, Meng Yang, Qinglin Pi, Qing Cao, Zhenggang Li, Yongliang Zhang, Xian-Bing Wang, Chenggui Han, Jialin Yu, Dawei Li

Plant physiology 2022 Jun 27

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Salicylic acid (SA) acts as a signaling molecule to perceive and defend against pathogen infections. Accordingly, pathogens evolve versatile strategies to disrupt the SA-mediated signal transduction, and how plant viruses manipulate the SA-dependent defense responses requires further characterization. Here, we show that barley stripe mosaic virus (BSMV) infection activates the SA-mediated defense signaling pathway and upregulates the expression of Nicotiana benthamiana thioredoxin h-type 1 (NbTRXh1). The γb protein interacts directly with NbTRXh1 in vivo and in vitro. The overexpression of NbTRXh1, but not a reductase-defective mutant, impedes BSMV infection, whereas low NbTRXh1 expression level results in increased viral accumulation. Similar with its orthologs in Arabidopsis (Arabidopsis thaliana), NbTRXh1 also plays an essential role in SA signaling transduction in N. benthamiana. To counteract NbTRXh1-mediated defenses, the BSMV γb protein targets NbTRXh1 to dampen its reductase activity, thereby impairing downstream SA defense gene expression to optimize viral cell-to-cell movement. We also found that NbTRXh1-mediated resistance defends against lychnis ringspot virus, beet black scorch virus, and beet necrotic yellow vein virus. Taken together, our results reveal a role for the multifunctional γb protein in counteracting plant defense responses and an expanded broad-spectrum antibiotic role of the SA signaling pathway. © American Society of Plant Biologists 2022. All rights reserved. For permissions, please email: journals.permissions@oup.com.

Citation

Zhihao Jiang, Xuejiao Jin, Meng Yang, Qinglin Pi, Qing Cao, Zhenggang Li, Yongliang Zhang, Xian-Bing Wang, Chenggui Han, Jialin Yu, Dawei Li. Barley stripe mosaic virus γb protein targets thioredoxin h-type 1 to dampen salicylic acid-mediated defenses. Plant physiology. 2022 Jun 27;189(3):1715-1727