MTO1-RESPONDING DOWN 1 (MRD1) is a transcriptional target of OZF1 for promoting salicylic acid-mediated defense in Arabidopsis.

Anupriya Singh, Akash Sharma, Nidhi Singh, Ashis Kumar Nandi

Plant cell reports 2022 May

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OZF1 promotes the transcription of MRD1, which is essential for SA-mediated defense against virulent and avirulent bacterial pathogens in Arabidopsis. Salicylic acid (SA) is critical for defense against biotrophic pathogens. A trans-activator protein NPR1 plays significant roles in SA-signaling. However, evidences suggest the existence of NPR1-independent pathways for SA signaling in plants. Previously, we reported Arabidopsis OXIDATION-RELATED ZN-FINGER PROTEIN1 (OZF1) as a positive regulator of NPR1-independent SA-signaling. However, the mechanism or components of OZF1-mediated SA signaling was not known. Through the analysis of differentially expressing genes, we report the identification of MTO1-RESPONDING DOWN 1 (MRD1) as a transcriptional target of OZF1. Expressions of MRD1 and its overlapping gene in Arabidopsis genome, HEI10 increase upon pathogen inoculation in an OZF1-dependent manner. Their mutants are susceptible to both virulent and avirulent bacterial pathogens and show compromised SA-mediated immunity. Overexpression of MRD1 but not the HEI10 rescues the loss-of-resistance phenotype of the ozf1 mutant. OZF1 physically associates at the MRD1 promoter area upon pathogen inoculation. Results altogether support that MRD1 is a transcriptional target of OZF1 for promoting SA-mediated defense in Arabidopsis. © 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.

Citation

Anupriya Singh, Akash Sharma, Nidhi Singh, Ashis Kumar Nandi. MTO1-RESPONDING DOWN 1 (MRD1) is a transcriptional target of OZF1 for promoting salicylic acid-mediated defense in Arabidopsis. Plant cell reports. 2022 May;41(5):1319-1328