Correlation Engine 2.0
Clear Search sequence regions

  • 4 and (2)
  • cytoplasm (1)
  • estrus (1)
  • female (1)
  • follicular phases (3)
  • lh cell (1)
  • LHB (3)
  • luteal phase (7)
  • mirnas (1)
  • molecular function (1)
  • mrna transcripts (1)
  • mrnas (2)
  • regulates (1)
  • research (1)
  • rna (7)
  • sheep (8)
  • Sizes of these terms reflect their relevance to your search.

    The pituitary gland directly regulates the reproduction of domestic animals. Research has increasingly focused on the potential regulatory mechanism of non-coding RNA in pituitary development. Little is known about the differential expression pattern of lncRNAs in Hu sheep, a famous sheep breed with high fecundity, and its role in the pituitary gland between the follicular phase and luteal phase. Herein, to identify the transcriptomic differences of the sheep pituitary gland during the estrus cycle, RNA sequencing (RNA-Seq) was performed. The results showed that 3529 lncRNAs and 16,651 mRNAs were identified in the pituitary gland. Among of them, 144 differentially expressed (DE) lncRNA transcripts and 557 DE mRNA transcripts were screened in the follicular and luteal phases. Moreover, GO and KEGG analyses demonstrated that 39 downregulated and 22 upregulated genes interacted with pituitary functions and reproduction. Lastly, the interaction of the candidate lncRNA XR_001039544.4 and its targeted gene LHB were validated in sheep pituitary cells in vitro. LncRNA XR_001039544.4 and LHB showed high expression levels in the luteal phase in Hu sheep. LncRNA XR_001039544.4 is mainly located in the cytoplasm, as determined by FISH analysis, indicating that XR_001039544.4 might act as competing endogenous RNAs for miRNAs to regulate LHB. LncRNA XR_001039544.4 knockdown significantly inhibited LH secretion and cell proliferation. LncRNA XR_001039544.4 may regulate the secretion of LH in the luteal-phase pituitary gland via affecting cell proliferation. Taken together, these findings provided genome-wide lncRNA- and mRNA-expression profiles for the sheep pituitary gland between the follicular and luteal phases, thereby contributing to the elucidation of the molecular mechanisms of pituitary function.


    Zhen Wan, Hua Yang, Yu Cai, Jianyu Ma, Peiyong Cheng, Zhibo Wang, Feng Wang, Yanli Zhang. Comparative Transcriptomic Analysis of Hu Sheep Pituitary Gland Prolificacy at the Follicular and Luteal Phases. Genes. 2022 Feb 27;13(3)

    Expand section icon Mesh Tags

    Expand section icon Substances

    PMID: 35327994

    View Full Text