Methods for vascularization and perfusion of tissue organoids.

Hannah A Strobel, Sarah M Moss, James B Hoying

Mammalian genome : official journal of the International Mammalian Genome Society 2022 Sep

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Tissue organoids or "mini organs" can be invaluable tools for understanding health and disease biology, modeling tissue dynamics, or screening potential drug candidates. Effective vascularization of these models is critical for truly representing the in vivo tissue environment. Not only is the formation of a vascular network, and ultimately a microcirculation, essential for proper distribution and exchange of oxygen and nutrients throughout larger organoids, but vascular cells dynamically communicate with other cells to modulate overall tissue behavior. Additionally, interstitial fluid flow, mediated by a perfused microvasculature, can have profound influences on tissue biology. Thus, a truly functionally and biologically relevant organoid requires a vasculature. Here, we review existing strategies for fabricating and incorporating vascular elements and perfusion within tissue organoids. © 2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.

Citation

Hannah A Strobel, Sarah M Moss, James B Hoying. Methods for vascularization and perfusion of tissue organoids. Mammalian genome : official journal of the International Mammalian Genome Society. 2022 Sep;33(3):437-450