Correlation Engine 2.0
Clear Search sequence regions

filter terms:
  • lipids (3)
  • mrna (3)
  • rna (1)
  • sirna (8)
  • Sizes of these terms reflect their relevance to your search.

    Single-stranded siRNA (ss-siRNA) refers to the antisense strand of siRNA, which plays the role of gene silencing. Since single-stranded RNA is unstable, the present study employed a homemade neutral cytidinyl/cationic lipid delivery system and chemical modifications to improve its stability. The results showed that with the aid of mixed lipids, ss-siRNA could knock down 40% of target mRNA at 25 nM. With 2'-F/2'-OMe, phosphorothioate and 5'-terminal phosphorylation, the optimized ss-siRNA could knock down 80% of target mRNA at 25 nM. Further knocking down AGO2, the ss-siRNAs could not effectively silence target mRNAs. Analysis of the biodistribution in vivo showed that ss-siRNA was less durable than siRNA, but spread more quickly to tissues. This study provides a safe and efficient ss-siRNA delivery and modification strategy, which lays the foundation for future works. Copyright © 2022 Li, Wang, Zhou, Wang, Guan and Yang.


    Zheng Li, Xixian Wang, Xinyang Zhou, Jie Wang, Zhu Guan, Zhenjun Yang. Optimization in Chemical Modification of Single-Stranded siRNA Encapsulated by Neutral Cytidinyl/Cationic Lipids. Frontiers in chemistry. 2022;10:843181

    PMID: 35345539

    View Full Text