Ethylene signaling modulates Arabidopsis thaliana nitrate metabolism.

Fiona Jamieson, Zhe Ji, Eric J Belfield, Zhong Jie Ding, Shao Jian Zheng, Nicholas P Harberd

Planta 2022 Mar 26

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Genetic analysis reveals a previously unknown role for ethylene signaling in regulating Arabidopsis thaliana nitrogen metabolism. Nitrogen (N) is essential for plant growth, and assimilation of soil nitrate (NO3-) and ammonium ions is an important route of N acquisition. Although N import and assimilation are subject to multiple regulatory inputs, the extent to which ethylene signaling contributes to this regulation remains poorly understood. Here, our analysis of Arabidopsis thaliana ethylene signaling mutants advances that understanding. We show that the loss of CTR1 function ctr1-1 mutation confers resistance to the toxic effects of the NO3- analogue chlorate (ClO3-), and reduces the activity of the nitrate reductase (NR) enzyme of NO3- assimilation. Our further analysis indicates that the lack of the downstream EIN2 component (conferred by novel ein2 mutations) suppresses the effect of ctr1-1, restoring ClO3- sensitivity and NR activity to normal. Collectively, our observations indicate an important role for ethylene signaling in regulating Arabidopsis thaliana NO3- metabolism. We conclude that ethylene signaling enables environmentally responsive coordination of plant growth and N metabolism. © 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.

Citation

Fiona Jamieson, Zhe Ji, Eric J Belfield, Zhong Jie Ding, Shao Jian Zheng, Nicholas P Harberd. Ethylene signaling modulates Arabidopsis thaliana nitrate metabolism. Planta. 2022 Mar 26;255(5):94