Callose Detection and Quantification at Plasmodesmata in Bryophytes.

Arthur Muller, Tomomichi Fujita, Yoan Coudert

Methods in molecular biology (Clifton, N.J.) 2022

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In bryophytes (i.e., mosses, liverworts, and hornworts), extant representatives of early land plants, plasmodesmata have been described in a wide range of tissues. Although their contribution to bryophyte morphogenesis remains largely unexplored, several recent studies have suggested that the deposition of callose around plasmodesmata might regulate developmental and physiological responses in mosses. In this chapter, we provide a protocol to image and quantify callose levels in the filamentous body of the model moss Physcomitrium (Physcomitrella) patens and discuss possible alternatives and pitfalls. More generally, this protocol establishes a framework to explore the distribution of callose in other bryophytes. © 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Citation

Arthur Muller, Tomomichi Fujita, Yoan Coudert. Callose Detection and Quantification at Plasmodesmata in Bryophytes. Methods in molecular biology (Clifton, N.J.). 2022;2457:177-187