Correlation Engine 2.0
Clear Search sequence regions


  • apoptosis (1)
  • bacteria (2)
  • biofilm (1)
  • dnase (1)
  • DNase IIs (1)
  • DNase2s (1)
  • dugesia (2)
  • endodeoxyribonucleases (2)
  • gram (1)
  • invertebrates (1)
  • phages (1)
  • planarian (7)
  • vertebrates (1)
  • Sizes of these terms reflect their relevance to your search.

    Deoxyribonuclease II (DNase II) has been found to regulate inflammation, autoimmunity and apoptosis in vertebrates and invertebrates. The strong capacity of degrading DNA makes DNase II play an important role in the immune process. Planarian has become one of the model references due to its strong immune system, the environment they live makes planarians face the threat of microorganisms and injury, the strong immune system can protect planarians from the threat of bacterial and infection. In this study, we found that there was DNase in the lysis buffer of planarians, then we acquired the sequence of DjDN2s (Dugesia japonica DNase2s) and confirmed the DjDN2s were conserved DNase IIs. The predicted structure showed the active sites and binding patterns of DjDN2s. Whole-mount in situ hybridization results showed DjDN2s mainly expressed in immune organs. Quantitative real-time PCR revealed that the expression of DjDN2s upregulated in varying degrees when got hurt and challenged with bacteria, and the knockdown of DjDN2s led to the slower repair of wound. The recombinant phages which take DjDN2 also had the ability to degrade DNA and clear young biofilm of Gram-negative bacteria. Collectively, DNase II of planarian might play a role in the antimicrobial response and wound-induced response. Copyright © 2022 Elsevier B.V. All rights reserved.

    Citation

    Zhe Sun, Tong Yu, Xiangyu Cao, Lili Gao, Qiuxiang Pang, Baohua Liu, Hongkuan Deng. Identification and characterization of Deoxyribonuclease II in planarian Dugesia japonica. Gene. 2022 Jun 05;826:146464

    Expand section icon Mesh Tags

    Expand section icon Substances


    PMID: 35358655

    View Full Text