Special AT-Rich Sequence-Binding Protein 1 Supports Survival and Maturation of Naive B Cells Stimulated by B Cell Receptors.

Takayuki Ozawa, Kentaro Fujii, Takao Sudo, Yukiko Doi, Ritsuko Nakai, Yasuhiro Shingai, Tomoaki Ueda, Yoshihiro Baba, Naoki Hosen, Takafumi Yokota

Journal of immunology (Baltimore, Md. : 1950) 2022 Apr 15

filter terms:

Epigenetic mechanisms underpin the elaborate activities of essential transcription factors in lymphocyte development. Special AT-rich sequence-binding protein 1 (SATB1) is a chromatin remodeler that orchestrates the spatial and temporal actions of transcription factors. Previous studies have revealed the significance of SATB1 in T cell lineage. However, whether and how SATB1 controls B cell lineage development is yet to be clarified. In this study, we show that SATB1 is an important factor during splenic B cell maturation. By analyzing SATB1/Tomato reporter mice, we determined the dynamic fluctuation of SATB1 expression in the B cell lineage. Although SATB1 expression decreased to minimal levels during B cell differentiation in the bone marrow, it resurged markedly in naive B cells in the spleen. The expression was dramatically downregulated upon Ag-induced activation. Splenic naive B cells were subdivided into two categories, namely SATB1high and SATB1-/low, according to their SATB1 expression levels. SATB1high naive B cells were less susceptible to death and greater proliferative than were SATB1-/low cells during incubation with an anti-IgM Ab. Additionally, SATB1high cells tended to induce the expression of MHC class II, CD86, and CD83. Accordingly, naive B cells from B lineage-specific SATB1 conditional knockout mice were more susceptible to apoptosis than that in the control group upon anti-IgM Ab stimulation in vitro. Furthermore, conditional knockout mice were less capable of producing Ag-specific B cells after immunization. Collectively, our findings suggest that SATB1 expression increases in naive B cells and plays an important role in their survival and maturation. Copyright © 2022 by The American Association of Immunologists, Inc.

Citation

Takayuki Ozawa, Kentaro Fujii, Takao Sudo, Yukiko Doi, Ritsuko Nakai, Yasuhiro Shingai, Tomoaki Ueda, Yoshihiro Baba, Naoki Hosen, Takafumi Yokota. Special AT-Rich Sequence-Binding Protein 1 Supports Survival and Maturation of Naive B Cells Stimulated by B Cell Receptors. Journal of immunology (Baltimore, Md. : 1950). 2022 Apr 15;208(8):1937-1946