β-Arrestin 2 acts an adaptor protein that facilitates viral replication in silkworm.

β-Arrestin 2 is known to be a widely distributed adaptor protein in mammals but its function has never been reported in Lepidoptera insects. Herein, the β-Arrestin 2 (BmArrestin 2) gene from silkworm was cloned and characterized. The spatiotemporal expression level of BmArrestin 2 was highest in the gonads at the 3rd day of 5th instar, whereas the highest and lowest abundance of BmArrestin 2 were identified in the tracheal and testis, respectively. BmArrestin 2 is mainly distributed in the cytoplasm. Furthermore, in BmN cells，overexpression of BmArrestin 2 promoted Bombyx mori nucleopolyhedrovirus (BmNPV) and B. mori cytoplasmic polyhedrosis virus (BmCPV) replication as the increment of the concentration of plasmid transfection, whereas silencing the gene with specific siRNA inhibited viral replication. Replication of BmNPV and BmCPV also was weakened using BmArrestin 2 antiserum as the increment of the concentration. Immunofluorescent staining revealed the invasion of recombinant BmNPV or BmCPV was decreased after blocking endogenous BmArrestin 2. On the other hand, BmArrestin 2 co-localizes with recombinant BmNPV and BmCPV virions in BmN cells. These results suggest that BmArrestin 2 may represent a novel target for antiviral strategies, as it is an adaptor protein that plays a key role in virus replication. Copyright © 2022 Elsevier B.V. All rights reserved.

Citation

Zi Liang, Renyu Xue, Xing Zhang, Manman Cao, Sufei Sun, Yunshan Zhang, Min Zhu, Ziyao Zhang, Kun Dai, Jun Pan, Guangli Cao, Chonglong Wang, Xiaolong Hu, Chengliang Gong. β-Arrestin 2 acts an adaptor protein that facilitates viral replication in silkworm. International journal of biological macromolecules. 2022 May 31;208:1009-1018

Mesh Tags

Substances

PMID: 35381288

search → result