Determination of tobramycin in eye drops with an open-source hardware ion mobility spectrometer.

The analysis of tobramycin was demonstrated successfully as an example for electrospray ionization on an open-source hardware ion mobility spectrometer. This instrument was assembled inexpensively in-house, and required only very few purpose-made components. The quantitative determination of tobramycin required 20 s for a reading. The calibration curve for the range from 50 to 200 μM was found to be linear with a correlation coefficient of r = 0.9994. A good reproducibility was obtained (3% relative standard deviation) and the limit of detection was determined as 8 μM. As the concentration of the active ingredient in the eye drops (ophthalmic solutions) is too high for the sensitivity of the instrument, the samples had to be diluted appropriately. © 2022. The Author(s).

Citation

Nattapong Chantipmanee, Peter C Hauser. Determination of tobramycin in eye drops with an open-source hardware ion mobility spectrometer. Analytical and bioanalytical chemistry. 2022 Jun;414(14):4059-4066

Mesh Tags

Substances

PMID: 35381854

search → result