PgtE Enzyme of Salmonella enterica Shares the Similar Biological Roles to Plasminogen Activator (Pla) in Interacting With DEC-205 (CD205), and Enhancing Host Dissemination and Infectivity by Yersinia pestis.

Frontiers in immunology 2022

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Yersinia pestis, the cause of plague, is a newly evolved Gram-negative bacterium. Through the acquisition of the plasminogen activator (Pla), Y. pestis gained the means to rapidly disseminate throughout its mammalian hosts. It was suggested that Y. pestis utilizes Pla to interact with the DEC-205 (CD205) receptor on antigen-presenting cells (APCs) to initiate host dissemination and infection. However, the evolutionary origin of Pla has not been fully elucidated. The PgtE enzyme of Salmonella enterica, involved in host dissemination, shows sequence similarity with the Y. pestis Pla. In this study, we demonstrated that both Escherichia coli K-12 and Y. pestis bacteria expressing the PgtE-protein were able to interact with primary alveolar macrophages and DEC-205-transfected CHO cells. The interaction between PgtE-expressing bacteria and DEC-205-expressing transfectants could be inhibited by the application of an anti-DEC-205 antibody. Moreover, PgtE-expressing Y. pestis partially re-gained the ability to promote host dissemination and infection. In conclusion, the DEC-205-PgtE interaction plays a role in promoting the dissemination and infection of Y. pestis, suggesting that Pla and the PgtE of S. enterica might share a common evolutionary origin. Copyright © 2022 Li, Ye, Zhao, Li, Zhu, Lv, Park, Zhang, Jiang, Yang, He, Cai, Zhang, Ding, Njiri, Tembo, Alkraiem, Li, Sun, Li, Yan, Kan, Huo, Klena, Skurnik, Anisimov, Gao, Han, Yang, Xiamu, Wang, Chen, Chai, Sun, Yuan and Chen.

Citation

Qiao Li, Chenglin Ye, Fei Zhao, Wenjin Li, Sizhe Zhu, Yin Lv, Chae Gyu Park, Yingmiao Zhang, Ling-Yu Jiang, Kun Yang, Yingxia He, Huahua Cai, Song Zhang, Hong-Hui Ding, Olivia Adhiambo Njiri, John Mambwe Tembo, Ayman Ahmad Alkraiem, An-Yi Li, Zi-Yong Sun, Wei Li, Mei-Ying Yan, Biao Kan, Xixiang Huo, John D Klena, Mikael Skurnik, Andrey P Anisimov, Xiaofang Gao, Yanping Han, Rui-Fu Yang, Xiding Xiamu, Yuanzhi Wang, Hongxiang Chen, Bao Chai, Yicheng Sun, Jingping Yuan, Tie Chen. PgtE Enzyme of Salmonella enterica Shares the Similar Biological Roles to Plasminogen Activator (Pla) in Interacting With DEC-205 (CD205), and Enhancing Host Dissemination and Infectivity by Yersinia pestis. Frontiers in immunology. 2022;13:791799