Chemiluminescence assay for kanamycin based on target recycling strategy.

A chemiluminescence (CL) sensing strategy for kanamycin residue detection in fish samples was established based on luminol-functionalized gold nanoparticles as CL nanoprobe materials combined with DNA hairpin structure and carboxyl-modified magnetic beads. Relying on nucleic acid amplification technology, the system can successfully realize the recycling of kanamycin, so that the biosensor can release a large number of luminol-functionalized gold nanoparticles with excellent CL performance even at a low residual levels of kanamycin. The biosensor strategy showed a good linear relationship with kanamycin in the range 0.09-130 nM, the detection limit was as low as 0.04 nM. This method proves the excellent performance of the sensing strategy and provides a low-cost and high-sensitivity CL analysis strategy for the detection of kanamycin and even other antibiotics. © 2022 John Wiley & Sons Ltd.

Citation

Liyuan Yu, Xiaoqian Zhang, Daobin Jin, Fangxu Lou, Jikuan Zhao, Xu Hun. Chemiluminescence assay for kanamycin based on target recycling strategy. Luminescence : the journal of biological and chemical luminescence. 2022 Jun;37(6):987-994

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PMID: 35411693

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