Split-GFP Complementation to Study the Nuclear Membrane Proteome Using Microscopy.

Shary N Shelton, Sarah E Smith, Sue L Jaspersen

Methods in molecular biology (Clifton, N.J.) 2022

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Defining the proteome of any given subcellular compartment provides insight into the activities and functions within that organelle. Understanding the composition of the nuclear envelope (NE) using traditional methods such as biochemical subcellular fractionation has been challenging due to the continuity of the NE and the endoplasmic reticulum. Here, we describe how split green fluorescent protein (split-GFP) was adapted to determine and define the NE proteome. This system is able to resolve protein topology and distinguish localization to the inner or outer nuclear membranes (INM or ONM). © 2022. Springer Science+Business Media, LLC, part of Springer Nature.

Citation

Shary N Shelton, Sarah E Smith, Sue L Jaspersen. Split-GFP Complementation to Study the Nuclear Membrane Proteome Using Microscopy. Methods in molecular biology (Clifton, N.J.). 2022;2502:205-213