Validation of Nuclear Pore Complex Protein-Protein Interactions by Transient Expression in Plants.

Fumika Ikeda, Kentaro Tamura

Methods in molecular biology (Clifton, N.J.) 2022

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The nuclear pore complex (NPC) is the largest protein complex, consisting of multiple copies of over 30 different nucleoporins. The interactions between the nucleoporins are critical elements for the NPC functions of the nuclear envelope in plant cells. In recent years, transient expression-based validations of protein-protein interactions have been widely used in plants. Bimolecular fluorescence complementation assay and coimmunoprecipitation assays are powerful tools to identify the molecules that interact with specific proteins. Here, as an example, we describe these techniques using nucleoporin protein interactions in plants. © 2022. Springer Science+Business Media, LLC, part of Springer Nature.

Citation

Fumika Ikeda, Kentaro Tamura. Validation of Nuclear Pore Complex Protein-Protein Interactions by Transient Expression in Plants. Methods in molecular biology (Clifton, N.J.). 2022;2502:235-243