Courtney Lancaster, Giulia Zavagno, James Groombridge, Adelaide Raimundo, David Weinkove, Tim Hawkins, Joanne Robson, Martin W Goldberg
Methods in molecular biology (Clifton, N.J.) 2022C. elegans is a well-characterized and relatively simple model organism, making it attractive for studying nuclear pore complex proteins in cell and developmental biology. C. elegans is transparent and highly amendable to genetic manipulation. Therefore, it is possible to generate fluorescently tagged proteins and combine this with various light microscopy techniques to study protein behavior in space and time. Here, we provide protocols to prepare both fixed and live C. elegans for confocal and light sheet microscopy. This enables the analysis of nuclear pore complex proteins from embryonic stages to the aging adult. © 2022. Springer Science+Business Media, LLC, part of Springer Nature.
Courtney Lancaster, Giulia Zavagno, James Groombridge, Adelaide Raimundo, David Weinkove, Tim Hawkins, Joanne Robson, Martin W Goldberg. Imaging Fluorescent Nuclear Pore Complex Proteins in C. elegans. Methods in molecular biology (Clifton, N.J.). 2022;2502:373-393
PMID: 35412251
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