Live-Cell Imaging of RNA Transport in Axons of Cultured Primary Neurons.

J Tabitha Hees, Angelika B Harbauer

Methods in molecular biology (Clifton, N.J.) 2022

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The use of fluorescent proteins has revolutionized the study of protein localization and transport. However, the visualization of other molecules and specifically RNA during live-cell imaging remains challenging. In this chapter, we provide guidance to the available methods, their advantages and drawbacks as well as provide a detailed protocol for the detection of RNA transport using the MS2/PP7-split-Venus system for background-free RNA imaging. © 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Citation

J Tabitha Hees, Angelika B Harbauer. Live-Cell Imaging of RNA Transport in Axons of Cultured Primary Neurons. Methods in molecular biology (Clifton, N.J.). 2022;2431:225-237