Determination of abacavir in biological objects for the purpose of chemical and toxicological analysis].

E A Illarionova, N V Chmelevskaya, Yu A Gonchikova, A E Mitina

Sudebno-meditsinskaia ekspertiza 2022

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Purpose - to develop a technique for isolating abacavir, as well as its detection and quantification in biological objects. Extraction of abacavir from centrifuges was carried out with chloroform at pH 8 in the presence of an electrolyte saturated solution of (NH4)2SO4 once for 3 minutes. The determination of abacavir in extracts from urine, saliva and liver was carried out by thin-layer chromatography in the system of ethyl acetate: trichloromethane: ammonia, a concentrated solution of 25% (17:4:1) ascending method, UV spectrophotometry in a medium of hydrochloric acid 0.1 M, where the absorption spectrum abacavir is characterized by an absorption maximum at a wavelength of 297±1 nm, high performance liquid chromatography, during which one peak with a retention time of 9.5 min was observed on the chromatogram of a standard sample, which coincided with the retention time of abacavir obtained after extraction from biological objects.

Citation

E A Illarionova, N V Chmelevskaya, Yu A Gonchikova, A E Mitina. Determination of abacavir in biological objects for the purpose of chemical and toxicological analysis]. Sudebno-meditsinskaia ekspertiza. 2022;65(2):29-33