Simultaneous quantification of trimethoprim metabolites in pediatric plasma.

The dual agent antibiotic, trimethoprim/sulfamethoxazole (TMP-SMX), has been prescribed to treat or prevent infections for over 50 years. However, there are no published validated analytical methods for the measurement of TMP metabolites in humans. We developed methodology enabling reliable quantification of TMP and 5 metabolites in human plasma. Chromatographic separation was achieved in less than 8 min using a biphenyl column. Analytes were detected in positive electrospray mode using a tandem Waters Xevo-TQ-XS mass spectrometer. Precision and accuracy values for all analytes were within 15% of nominal values during assay validation. Copyright © 2022 Elsevier B.V. All rights reserved.

Citation

Robert T Tessman, Whitney Nolte, Paul Toren, Kim Gibson, Linda Vu, Jennifer Goldman. Simultaneous quantification of trimethoprim metabolites in pediatric plasma. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences. 2022 May 15;1198:123232

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PMID: 35428009

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