Detection of SARS-CoV-2 peptide-specific antibodies in Syrian hamster serum by ELISA.

Huy Quang Quach, Inna G Ovsyannikova, Gregory A Poland, Richard B Kennedy

Journal of immunological methods 2022 Jun

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Golden Syrian hamsters are increasingly used as a permissive animal model for SARS-CoV-2 virus studies, but the lack of immunological assays and other immunological reagents for hamsters limits its full potential. Herein, we developed an ELISA method to detect antibodies specific to peptides and proteins derived from SARS-CoV-2 virus in immunized golden Syrian hamsters. Under optimized conditions, this assay quantitates antibodies specific for individual viral peptides, peptide pools, and proteins. Hence, this ELISA method allows investigators to quantitatively assess humoral immune responses at the peptide and protein levels and has potential application in the development of peptide-based vaccines to be tested in hamsters. Copyright © 2022 Elsevier B.V. All rights reserved.

Citation

Huy Quang Quach, Inna G Ovsyannikova, Gregory A Poland, Richard B Kennedy. Detection of SARS-CoV-2 peptide-specific antibodies in Syrian hamster serum by ELISA. Journal of immunological methods. 2022 Jun;505:113275