A Real-Time Detection Device for the Rapid Quantification of Skin Casual Sebum Using the Oil Red O Staining Method.

Kung Ahn, Sangjin Han, Kyeongeui Yun, Wooseok Lee, Dong-Geol Lee, So Min Kang, Young-Bong Choi, Kyudong Han, Yong Ju Ahn

Sensors (Basel, Switzerland) 2022 Apr 14

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The human skin sebum suggests that it (along with other epidermal surface lipids) plays a role in skin barrier formation, the moderation of cutaneous inflammation, and antimicrobial defense. Various methods have been developed for collecting and measuring skin sebum. We tested methods of detection using "color intensity", by staining the skin casual sebum. This process was conducted in three steps; first, the selection of materials for sebum collection; second, staining the collected sebum; third, the development of a device that can measure the level of stained sebum. A plastic film was used to effectively collect sebum that increased with the replacement time of the sebum. In addition, the collected sebum was stained with Oil Red O (ORO) and checked with RGB; as a result, the R2 value was higher than 0.9. It was also confirmed that the correlation value was higher than 0.9 in the comparison result with Sebumeter®, which is a common standard technology. Finally, it was confirmed that the R2 value was higher than 0.9 in the detection value using the sensor. In conclusion, we have proven the proof of concept (PoC) for this method, and we would like to introduce an effective sebum measurement method that differs from the existing method.

Citation

Kung Ahn, Sangjin Han, Kyeongeui Yun, Wooseok Lee, Dong-Geol Lee, So Min Kang, Young-Bong Choi, Kyudong Han, Yong Ju Ahn. A Real-Time Detection Device for the Rapid Quantification of Skin Casual Sebum Using the Oil Red O Staining Method. Sensors (Basel, Switzerland). 2022 Apr 14;22(8)