Correlation Engine 2.0
Clear Search sequence regions

  • biosynthesis (1)
  • cao (1)
  • cheA (1)
  • chemotaxis (1)
  • cheX (1)
  • cheY (1)
  • flaD (2)
  • flagellum (1)
  • flgB (1)
  • flgBCDGKLN (1)
  • gel shift analysis (1)
  • genes expressed (1)
  • motA (1)
  • mycelium (2)
  • PHO (1)
  • PhoP (7)
  • phoR (1)
  • phosphate (2)
  • represses (1)
  • respond (1)
  • signal (1)
  • spores (2)
  • ssgB (2)
  • swrD (1)
  • Sizes of these terms reflect their relevance to your search.

    PhoPR is an important two-component signal transduction system (TCS) for microorganisms to sense and respond to phosphate limitation. Although the response regulator PhoP controls morphological development and secondary metabolism in various Streptomyces species, the function of PhoP in Actinosynnema pretiosum remains unclear. In this study, we showed that PhoP significantly represses the morphological development of the A. pretiosum X47 strain. Production of aerial mycelium and spore formation occurred much earlier in the ΔphoP strain than in X47 during growth on ISP2 medium. Transcription analysis indicated that 222 genes were differentially expressed in ∆phoP compared to strain X47. Chemotaxis genes (cheA, cheW, cheX, and cheY); flagellum biosynthesis and motility genes (flgBCDGKLN, flaD, fliD-R, motA, and swrD); and differentiation genes (whiB and ssgB) were significantly upregulated in ∆phoP. Gel-shift analysis indicated that PhoP binds to the promoters of flgB, flaD, and ssgB genes, and PHO box-like motif with the 8-bp conserved sequence GTTCACGC was identified. The transcription of phoP/phoR of X47 strain was induced at low phosphate concentration. Our results demonstrate that PhoP is a negative regulator that controls the morphological development of A. pretiosum X47 by repressing the transcription of differentiation genes. Copyright © 2022 Zhang, Zhang, Liu, Fu, Zong, Ma and Cao.


    Peipei Zhang, Kunyu Zhang, Yayu Liu, Jiafang Fu, Gongli Zong, Xin Ma, Guangxiang Cao. Deletion of the Response Regulator PhoP Accelerates the Formation of Aerial Mycelium and Spores in Actinosynnema pretiosum. Frontiers in microbiology. 2022;13:845620

    PMID: 35464974

    View Full Text