Spontaneous Alignment of Microtubules Via Tubulin Polymerization in a Narrow Space Under a Temperature Gradient.

In this chapter, protocols for spontaneous alignment of microtubules (MTs), such as helices and spherulites, via tubulin polymerization in a narrow space and under a temperature gradient are presented for tubulin solutions and tubulin-polymer mixtures. These protocols provide an easy route for hierarchical MT assembly and may extend our current understanding of cytoskeletal protein self-assembly under dissipative conditions. © 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

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Kazuhiro Shikinaka. Spontaneous Alignment of Microtubules Via Tubulin Polymerization in a Narrow Space Under a Temperature Gradient. Methods in molecular biology (Clifton, N.J.). 2022;2430:185-192

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PMID: 35476332

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