Lactate induces vascular permeability via disruption of VE-cadherin in endothelial cells during sepsis.

Kun Yang, Min Fan, Xiaohui Wang, Jingjing Xu, Yana Wang, P Spencer Gill, Tuanzhu Ha, Li Liu, Jennifer V Hall, David L Williams, Chuanfu Li

Science advances 2022 Apr 29

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Circulating lactate levels are a critical biomarker for sepsis and are positively correlated with sepsis-associated mortality. We investigated whether lactate plays a biological role in causing endothelial barrier dysfunction in sepsis. We showed that lactate causes vascular permeability and worsens organ dysfunction in CLP sepsis. Mechanistically, lactate induces ERK-dependent activation of calpain1/2 for VE-cadherin proteolytic cleavage, leading to the enhanced endocytosis of VE-cadherin in endothelial cells. In addition, we found that ERK2 interacts with VE-cadherin and stabilizes VE-cadherin complex in resting endothelial cells. Lactate-induced ERK2 phosphorylation promotes ERK2 disassociation from VE-cadherin. In vivo suppression of lactate production or genetic depletion of lactate receptor GPR81 mitigates vascular permeability and multiple organ injury and improves survival outcome in polymicrobial sepsis. Our study reveals that metabolic cross-talk between glycolysis-derived lactate and the endothelium plays a critical role in the pathophysiology of sepsis.

Citation

Kun Yang, Min Fan, Xiaohui Wang, Jingjing Xu, Yana Wang, P Spencer Gill, Tuanzhu Ha, Li Liu, Jennifer V Hall, David L Williams, Chuanfu Li. Lactate induces vascular permeability via disruption of VE-cadherin in endothelial cells during sepsis. Science advances. 2022 Apr 29;8(17):eabm8965