Athéna Labeau, Luc Fery-Simonian, Alain Lefevre-Utile, Marie Pourcelot, Lucie Bonnet-Madin, Vassili Soumelis, Vincent Lotteau, Pierre-Olivier Vidalain, Ali Amara, Laurent Meertens
Cell reports 2022 Apr 26Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of the COVID-19 pandemic, which has led to a devastating global health crisis. The emergence of variants that escape neutralizing responses emphasizes the urgent need to deepen our understanding of SARS-CoV-2 biology. Using a comprehensive identification of RNA-binding proteins (RBPs) by mass spectrometry (ChIRP-MS) approach, we identify 107 high-confidence cellular factors that interact with the SARS-CoV-2 genome during infection. By systematically knocking down their expression in human lung epithelial cells, we find that the majority of the identified RBPs are SARS-CoV-2 proviral factors. In particular, we show that HNRNPA2B1, ILF3, QKI, and SFPQ interact with the SARS-CoV-2 genome and promote viral RNA amplification. Our study provides valuable resources for future investigations into the mechanisms of SARS-CoV-2 replication and the identification of host-centered antiviral therapies. Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.
Athéna Labeau, Luc Fery-Simonian, Alain Lefevre-Utile, Marie Pourcelot, Lucie Bonnet-Madin, Vassili Soumelis, Vincent Lotteau, Pierre-Olivier Vidalain, Ali Amara, Laurent Meertens. Characterization and functional interrogation of the SARS-CoV-2 RNA interactome. Cell reports. 2022 Apr 26;39(4):110744
PMID: 35477000
View Full Text