Pathogenic detection and molecular tracing of a bacillary dysentery outbreak by Shigella sonnei in Huainan city].

J Liu, Y Sun, F R Zhang, X P Zeng, L J Wang, M Y Zhu, S J Sun, Shoujie Yu

Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine] 2022 Apr 06

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Objective: To understand the virulence gene and drug resistance profile of Shigella sonnei outbreak in Huainan city, and conduct pathogenic traceability analysis. Methods: Water samples and feces related to an infectious diarrhea outbreak in Huainan city in August 2020 were collected for multiple pathogen detection. Virulence gene, drug sensitivity, pulse-field gel electrophoresis and whole genome sequencing of Shigella isolates were analyzed respectively. Results: 38 strains of Shigella sonnei were detected in 56 samples of mucilage feces with a positive rate 67.86%, and all serotypes were Shigella sonnei Phase I. Three strains of Shigella sonnei were detected by fluorescence PCR in the Gram-negative (GN) bacterial enrichment solution of terminal water and well water. Virulence genes were ipaH positive (38), ipaH/ial (31) and ipaH/ial/sen positive (1), respectively. The drug resistance spectrum showed that 9 of 14 antibiotics were 100% resistant, and only imipenem, chloramphenicol, ceftazidime and ciprofloxacin were effective drugs. XbaⅠ restriction enzyme map type of 36 isolates was completely consistent, and the ST type analysis of 3 strains was ST152. Whole genome sequencing and analysis verified that the outbreak was caused by a single clonal group of strains, and revealed that the isolates of the outbreak were clustered into a large cluster with 3 Chinese strains and 1 Korean strain in the database, far away from the strains of other countries. Conclusion: The outbreak is caused by a single clone of Shigella sonnei, which are low virulence strains and have multiple drug resistance.

Citation

J Liu, Y Sun, F R Zhang, X P Zeng, L J Wang, M Y Zhu, S J Sun, Shoujie Yu. Pathogenic detection and molecular tracing of a bacillary dysentery outbreak by Shigella sonnei in Huainan city]. Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]. 2022 Apr 06;56(4):437-442