Cyclotide Isolation from Psychotria brachyceras and Psychotria leiocarpa.

Hélio Nitta Matsuura

Methods in molecular biology (Clifton, N.J.) 2022

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Cyclotides are small circular peptides carrying an array of interesting biological activities and also showing interesting features for storage and bioavailability. Here, an optimized method to isolate cyclotides from two species of Psychotria, P. brachyceras and P. leiocarpa, that can be integrally performed (to isolate cyclotides) or used in part (to obtain cyclotide-rich extracts), is described. In general this protocol can be applied for cyclotide isolation from any species, taking into account potential minor adaptations for the particularities of specific cases. © 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Citation

Hélio Nitta Matsuura. Cyclotide Isolation from Psychotria brachyceras and Psychotria leiocarpa. Methods in molecular biology (Clifton, N.J.). 2022;2469:165-181