Bioproduction of eriodictyol by Escherichia coli engineered co-culture.

Eriodictyol (ED) is a flavonoid in the flavanones subclass. It is abundantly present in a wide range of medicinal plants, citrus fruits, and vegetables. In addition, ED owns numerous importantly medicinal bioactivities such as inhibition of proliferation, metastasis and induction of apoptosis in glioma cells or inhibition of glioblastoma migration, and invasion. This study described the heterologous production of ED by E. coli based co-culture engineering system from the simple carbon substrate D-glucose. Two E. coli strains were engineered and functioned as constitutive components of biological system. Specifically, the first strain (upstream module) contained genes for synthesis of p-coumaric acid (pCA) from D-glucose. And, the second strain (downstream module) consisted of genes for the synthesis of ED from pCA. The highest yield in ED production was achieved 51.5 ± 0.4 mg/L using stepwise optimal culture conditions, while monoculture was achieved 21.3 ± 0.2 mg/L only. In conclusion, co-culture was the most efficient alternative approach for the synthesis of ED and other natural products. © 2022. The Author(s), under exclusive licence to Springer Nature B.V.

Citation

Nguyen Huy Thuan, Vinay Bharadwaj Tatipamula, Tran Thanh Viet, Nguyen Quang Duc Tien, Nguyen Hoang Loc. Bioproduction of eriodictyol by Escherichia coli engineered co-culture. World journal of microbiology & biotechnology. 2022 May 16;38(7):112

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PMID: 35570219

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