HDAC6 modulates myofibril stiffness and diastolic function of the heart.

Passive stiffness of the heart is determined largely by extracellular matrix and titin, which functions as a molecular spring within sarcomeres. Titin stiffening is associated with the development of diastolic dysfunction (DD), while augmented titin compliance appears to impair systolic performance in dilated cardiomyopathy. We found that myofibril stiffness was elevated in mice lacking histone deacetylase 6 (HDAC6). Cultured adult murine ventricular myocytes treated with a selective HDAC6 inhibitor also exhibited increased myofibril stiffness. Conversely, HDAC6 overexpression in cardiomyocytes led to decreased myofibril stiffness, as did ex vivo treatment of mouse, rat, and human myofibrils with recombinant HDAC6. Modulation of myofibril stiffness by HDAC6 was dependent on 282 amino acids encompassing a portion of the PEVK element of titin. HDAC6 colocalized with Z-disks, and proteomics analysis suggested that HDAC6 functions as a sarcomeric protein deacetylase. Finally, increased myofibril stiffness in HDAC6-deficient mice was associated with exacerbated DD in response to hypertension or aging. These findings define a role for a deacetylase in the control of myofibril function and myocardial passive stiffness, suggest that reversible acetylation alters titin compliance, and reveal the potential of targeting HDAC6 to manipulate the elastic properties of the heart to treat cardiac diseases.

Citation

Ying-Hsi Lin, Jennifer L Major, Tim Liebner, Zaynab Hourani, Joshua G Travers, Sara A Wennersten, Korey R Haefner, Maria A Cavasin, Cortney E Wilson, Mark Y Jeong, Yu Han, Michael Gotthardt, Scott K Ferguson, Amrut V Ambardekar, Maggie Py Lam, Chunaram Choudhary, Henk L Granzier, Kathleen C Woulfe, Timothy A McKinsey. HDAC6 modulates myofibril stiffness and diastolic function of the heart. The Journal of clinical investigation. 2022 May 16;132(10)

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PMID: 35575093

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