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Gut microbiota is a complex ecosystem composed by trillions of microorganisms that are crucial for human health or disease status. Currently, there are two methodological options to explore its complexity: metagenomics and culturomics. Culturomics is an approach that uses multiple culture conditions (days of incubation, enrichment factors and growth temperature) and MALDI-TOF mass spectrometry for the identification of bacterial species and sequencing when this method fails. In this paper, we describe how Colturomic's protocol has allowed the first isolation in human sample of Rummeliibacillus suwonensis, a Gram positive, facultative anaerobe bacterium. The bacterium was isolated from feces of a 69 years old male with amyotrophic lateral sclerosis (ALS) recruited for a clinical trial assessing safety and efficacy of fecal microbiota transplantation in ALS. The first isolation of the microorganism dates back to 2013 from the soil of a South Korean mountain area. In this report, morphological description, biochemical characterization and antibiotic susceptibility tests were performed to outline the bacterial properties. © 2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.


Gianluca Quaranta, Jessica Mandrioli, Stefano Bibbò, Alessandra Guarnaccia, Giovanni Fancello, Cecilia Simonini, Amedeo Amedei, Elena Niccolai, Giulia Nannini, Giovanni Cammarota, Maurizio Sanguinetti, Luca Masucci. Rummeliibacillus suwonensis: First Time Isolation from Human Feces by Culturomics. Current microbiology. 2022 May 20;79(7):197

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PMID: 35595837

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