Multifaceted Cargo Recruitment and Release from Artificial Membraneless Organelles.

Jianhui Liu, Fariza Zhorabek, Tianfu Zhang, Jacky W Y Lam, Ben Zhong Tang, Ying Chau

Small (Weinheim an der Bergstrasse, Germany) 2022 Jun

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Liquid-liquid phase separation (LLPS) drives membraneless organelles (MLOs) formation for organizing biomolecules. Artificial MLOs (AMLOs) have been constructed mostly via the LLPS of engineered proteins capable of regulating limited types of biomolecules. Here, leveraging a minimalist AMLO, driven by LLPS of polymer-oligopeptide hybrids, enrichment, recruitment, and release of multifaceted cargoes are quantitatively shown, including small fluorescent molecules, fluorophore-containing macromolecules, proteins, DNAs, and RNAs. Cargoes show up to 105 -fold enrichment, whilst recruitment and release are triggered by variations of temperature, pH, and/or ionic strength. Also, the first efficacious, rapid, and reversible control of aggregation-induced emission with over 30 folds of modulation of overall fluorescence intensity is achieved, by intensifying the aggregation of luminogens in AMLO. The AMLO is a simple yet versatile platform for potential drug delivery and biosensor applications. © 2022 Wiley-VCH GmbH.

Citation

Jianhui Liu, Fariza Zhorabek, Tianfu Zhang, Jacky W Y Lam, Ben Zhong Tang, Ying Chau. Multifaceted Cargo Recruitment and Release from Artificial Membraneless Organelles. Small (Weinheim an der Bergstrasse, Germany). 2022 Jun;18(25):e2201721