Correlation Engine 2.0
Clear Search sequence regions

  • collagen (12)
  • donors (12)
  • graft (1)
  • humans (1)
  • lung diseases (1)
  • Sizes of these terms reflect their relevance to your search.

    To evaluate the initial use of label-free second harmonic generation (SHG) imaging with two-photon excitation (2PE) auto-fluorescence in multiphoton microscopy (MPM) for the quantification of collagen/fibrosis on preimplantation biopsies of extended criteria donors (ECD). Twenty preimplantation core biopsies were extracted from 10 donor kidney samples, of which originated from seven donors. Kidney Donor Profile Index (KDPI) and Remuzzi scores of biopsies were calculated. Collagen parameters measured included quantification by the Collagen Area Ratio in Total Tissue (CART) and qualitative measurements by Collagen Reticulation Index (CRI). Biopsies classified with > 85% KDPI scores had significantly higher CART (p = .011) and lower CRI values (p = .025) than biopsies with ≤ 85% KDPI scores. Increase in CRI values correlated significantly with rise in recipient creatinine levels 1-year post-transplant (p = .027; 95% CI: 4.635-66.797). MPM is an evolving technology that enables the quantification of the amount (CART) and quality (CRI) of collagen deposition in unstained preimplantation biopsies of donor kidneys stratified by KDPI scores. This initial evaluation found significant differences in both parameters between donor kidneys with more or less than 85% KDPI. © 2022 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.


    Wei Zheng So, Rachel Zui Chih Teo, Li Yin Ooi, Benjamin Yen Seow Goh, Jirong Lu, Anantharaman Vathsala, Thomas Paulraj Thamboo, Ho Yee Tiong. Multi-photon microscopy for the evaluation of interstitial fibrosis in extended criteria donor kidneys: A proof-of-concept study. Clinical transplantation. 2022 Aug;36(8):e14717

    Expand section icon Mesh Tags

    Expand section icon Substances

    PMID: 35598116

    View Full Text