Facile purification of active recombinant mouse cytosolic carboxypeptidase 6 from Escherichia coli.

Xinyu Guo, Ruixue Wang, Ruifang Ma, Xiaona Fan, Yan Gao, Xiangyang Zhang, Zhiguang Yuchi, Hui-Yuan Wu

Protein expression and purification 2022 Sep

filter terms:

CCP6 is a member of cytosolic carboxypeptidases (CCPs) family, an eraser of a reversible protein posttranslational modification - polyglutamylation, and represents a potential therapeutic target. Currently, production of CCPs mainly depends on eukaryotic expression system, which is time-consuming and costly. Here, we reported that mouse origin full-length CCP6 can be successfully expressed in the soluble fraction of bacteria ArcticExpress (DE3) strain. However, the recombinant mCCP6 was initially co-purified with Cpn60 in a stoichiometric ratio of roughly 1:7 and exhibited no enzyme activity. When coupled with a step to promote the release of the substrate protein from the chaperonins by treatment with ATP/Mg2+/K+, the recombinant CCP6 with deglutamylation activity was obtained, though still partially associated with Cpn60. This is the first report, to our knowledge, that the successful expression and purification of active recombinant mammalian CCPs using a bacterial system was achieved. Copyright © 2022. Published by Elsevier Inc.

Citation

Xinyu Guo, Ruixue Wang, Ruifang Ma, Xiaona Fan, Yan Gao, Xiangyang Zhang, Zhiguang Yuchi, Hui-Yuan Wu. Facile purification of active recombinant mouse cytosolic carboxypeptidase 6 from Escherichia coli. Protein expression and purification. 2022 Sep;197:106112