In situ Nuclear Matrix preparation in Drosophila melanogaster enabling genetic analysis of the nuclear architecture.

Rashmi U Pathak, Ashish Bihani, Rahul Sureka, Rakesh K Mishra

STAR protocols 2022 Jun 17

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Nuclear Matrix (NuMat) is a biochemically defined entity that provides us with a snapshot of the features of the nuclear architecture. Here, we present a protocol to isolate and visualize NuMat in situ in the intact embryo or tissues of Drosophila melanogaster and its applications. We remove the chromatin to reveal underlying nuclear architectural components in organismal context. This protocol couples the power of Drosophila genetics with cell biological observation of the nuclear architecture. For complete details on the use and execution of this protocol, please refer to Pathak et al. (2022), Sureka et al. (2018), and Pathak et al. (2013). © 2022 The Author(s).

Citation

Rashmi U Pathak, Ashish Bihani, Rahul Sureka, Rakesh K Mishra. In situ Nuclear Matrix preparation in Drosophila melanogaster enabling genetic analysis of the nuclear architecture. STAR protocols. 2022 Jun 17;3(2):101394