Actomyosin contractility and buckling of microtubules in nucleation, growth and disassembling of focal adhesions.

S Palumbo, E Benvenuti, M Fraldi

Biomechanics and modeling in mechanobiology 2022 Aug

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Building up and maintenance of cytoskeletal structure in living cells are force-dependent processes involving a dynamic chain of polymerization and depolymerization events, which are also at the basis of cells' remodelling and locomotion. All these phenomena develop by establishing cell-matrix interfaces made of protein complexes, known as focal adhesions, which govern mechanosensing and mechanotransduction mechanisms mediated by stress transmission between cell interior and external environment. Within this framework, by starting from a work by Cao et al. (Biophys J 109:1807-1817, 2015), we here investigate the role played by actomyosin contractility of stress fibres in nucleation, growth and disassembling of focal adhesions. In particular, we propose a tensegrity model of an adherent cell incorporating nonlinear elasticity and unstable behaviours, which provides a new kinematical interpretation of cellular contractile forces and describes how stress fibres, microtubules and adhesion plaques interact mechanobiologically. The results confirm some experimental evidences and suggest how the actomyosin contraction level could be exploited by cells to actively control their adhesion, eventually triggering cytoskeleton reconfigurations and migration processes observed in both physiological conditions and diseases. © 2022. The Author(s).

Citation

S Palumbo, E Benvenuti, M Fraldi. Actomyosin contractility and buckling of microtubules in nucleation, growth and disassembling of focal adhesions. Biomechanics and modeling in mechanobiology. 2022 Aug;21(4):1187-1200