Native ambient mass spectrometry of intact protein assemblies directly from Escherichia coli colonies.

Yuying Du, Robin C May, Helen J Cooper

Chemical communications (Cambridge, England) 2022 Jun 14

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Here, we demonstrate that by combining electroporation with native ambient mass spectrometry, it is possible to detect intact non-covalent protein complexes directly from bacterial colonies growing on agar. Homodimers HdeA and HdeB were identified, together with the 50 kDa Mn-bound superoxide dismutase homodimer, in addition to some previously undetected monomeric proteins.

Citation

Yuying Du, Robin C May, Helen J Cooper. Native ambient mass spectrometry of intact protein assemblies directly from Escherichia coli colonies. Chemical communications (Cambridge, England). 2022 Jun 14;58(48):6857-6860