Generation of MiRNA sponge constructs targeting multiple MiRNAs.

MicroRNAs(miRNAs) are key regulators of gene expression in plants, animals and some viruses. Hence, alteration of miRNA levels in cells or tissues is common for miRNA studies. Loss-of-function of miRNA can be achieved using antisense oligonucleotides, sponges and gene knockout models. Here, we showed an efficient, rational and economical way to construct multi-targeted miRNA sponges with desired copies. Four copies of miRNA sponge are used as "building-blocks". These building-blocks, which can target same miRNA or different miRNAs, are linked together through ligation. Each time of ligation can double the number of sponge copies. In this way, we constructed lentivirus vectors harboring sponges targeting miR-21, miR-31 and miR-155 and the combination of two miRNA sponges can inhibit cancer cell growth significantly. © 2022 The Authors. Journal of Clinical Laboratory Analysis published by Wiley Periodicals LLC.

Citation

Junjin Jie, Danni Liu, Yifan Wang, Qiong Wu, Tingting Wu, Rong Fang. Generation of MiRNA sponge constructs targeting multiple MiRNAs. Journal of clinical laboratory analysis. 2022 Jul;36(7):e24527

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PMID: 35666624

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