Hybridoma Screening by Antibody Capture: Immunohistochemistry.

Edward A Greenfield

Cold Spring Harbor protocols 2022 Jun 07

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To determine the subcellular location of an antigen, hybridoma tissue culture supernatants can be screened using immunohistochemistry. For antibodies to have access to antigens in fixed and embedded tissue sections, the paraffin must be removed and the tissue must be rehydrated and digested before immunohistochemical staining. © 2022 Cold Spring Harbor Laboratory Press.

Citation

Edward A Greenfield. Hybridoma Screening by Antibody Capture: Immunohistochemistry. Cold Spring Harbor protocols. 2022 Jun 07;2022(5):Pdb.prot103119