MiR-663a Inhibits Radiation-Induced Epithelium-to-Mesenchymal Transition by Targeting TGF-β1.

Pei Qu, Zhi Ang Shao, Bing Wang, Jin Peng He, Ya Nan Zhang, Wen Jun Wei, Jun Rui Hua, Heng Zhou, Dong Lu, Nan Ding, Ju Fang Wang

Biomedical and environmental sciences : BES 2022 May 20

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miR-663a has been reported to be downregulated by X-ray irradiation and participates in radiation-induced bystander effect via TGF-β1. The goal of this study was to explore the role of miR-663a during radiation-induced Epithelium-to-mesenchymal transition (EMT). TGF-β1 or IR was used to induce EMT. After miR-663a transfection, cell migration and cell morphological changes were detected and the expression levels of miR-663a, TGF-β1, and EMT-related factors were quantified. Enhancement of cell migration and promotion of mesenchymal changes induced by either TGF-β1 or radiation were suppressed by miR-663a. Furthermore, both X-ray and carbon ion irradiation resulted in the upregulation of TGF-β1 and downregulation of miR-663a, while the silencing of TGF-β1 by miR-663a reversed the EMT process after radiation. Our findings demonstrate an EMT-suppressing effect by miR-663a via TGF-β1 in radiation-induced EMT. Copyright © 2022 The Editorial Board of Biomedical and Environmental Sciences. Published by China CDC. All rights reserved.

Citation

Pei Qu, Zhi Ang Shao, Bing Wang, Jin Peng He, Ya Nan Zhang, Wen Jun Wei, Jun Rui Hua, Heng Zhou, Dong Lu, Nan Ding, Ju Fang Wang. MiR-663a Inhibits Radiation-Induced Epithelium-to-Mesenchymal Transition by Targeting TGF-β1. Biomedical and environmental sciences : BES. 2022 May 20;35(5):437-447